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In one study, Cunningham and colleagues (2016) assigned experienced e-cigarette users to either a first-generation device (Vype Reload, classic flavor bold containing 4.5 percent nicotine by volume) (n = 32) or to a button-activated, variable-voltage e-cigarette that uses prefilled cartridges containing e-liquid (Vype ePen with 3.0 percent nicotine by volume) (n = 28)